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Specimen Collection & Handling

Our Specimen Collection & Handling Guide
Supplies Types of Specimen Collection Containers General Specimen Preparation General Sample Storage Instructions on Collection of Blood Cultures Collection and Transportation of Microbiology Specimens Tips Regarding Sample Collection Conditions for Medical Centre In-Clinic Phlebotomy Services for Tests with Special Sample Collection Requirement

Types of Specimen Collection Containers

Blood Collection Containers – Common Tubes

Please refer to our Order of Draw posters provided.

Obtain a hard copy poster for your clinic by contacting your respective account managers or the Call Centre.

Order of Draw - Vacutainer Method Order of Draw - Wingset Method Order of Draw - Syringe Method

Note: Failure to fulfill the requirements stated may result in specimen rejection.

Other Specimen Collection Containers
TYPE OF CONTAINERS/DESCRIPTIONCOMMENTS
eSwab with liquid Amies medium (sterile applicator swab with flocked nylon fiber tip)

For bacteriological culture from all sites.

It contains a special liquid transport medium which will maintain micro-organism viability but does not promote growth of micro-organisms.

Pink cap eSwab is suitable for both aerobic & anaerobic organisms.

Double eSwab (flocked swabs with Amies medium)
  1. Pink eSwab - Sample axilla and groin, swirl in Amies media and discard swab
  2. White eSwab - Sample the anterior nares, break swab and place it in the tube of Amies media
For Methicillin-resistant Staphylococcus aureus (MRSA) PCR - XPERT test.
DRY SWAB without Transport Media

Sterile cotton swab attached to plastic applicator stick

For Chlamydia Trachomatis PCR and Neisseria Gonorrhoeae PCR test.
SINGLE eSWAB (flocked swabs with Liquid Amies medium) For Vancomycin-resistant enterococci (VRE) PCR - XPERT and Carbapenem-resistant Organisms (CRO) PCR - XPERT Tests.
STOOL CONTAINER

Screw cap (brown) plastic container with attached spatula

For collecting stool specimen for routine examination and culture.
URINE CONTAINER, Sterile (UNIVERSAL CONTAINER)

Sterile plastic container with screw cap

For routine urinalysis and urine culture.

Also suitable for collecting body fluids and sputum for routine examination or culture.

Cytology / Histology
TYPE OF CONTAINERS/DESCRIPTIONCOMMENTS
CONTAINER, with Formalin

Clean container (50mL) half-filled with formalin

Supplied for transporting small histopathology specimens.

GLASS SLIDE

Rectangular 3”x1” glass slide with frosted end

Supplied for collecting cytology smears only. Please write patient’s name on the frosted end of slide in pencil.
SLIDE HOLDER

Blue or white plastic holder for 2 slide wells.

Supplied with spray fixative

Supplied for holding cytology glass slides only.
For Genetics Department
TYPE OF SUPPLIESCOMMENTS
BOTTLE OF TRANSPORT MEDIUM

For QF-PCR and Karyotyping on Chorionic Villus or Products of Conception

BOTTLE OF TRANSPORT MEDIUM For Bone Marrow Karyotyping

MOTTLED BLACK/TAN

Cell-Free DNA

BCT has chemical additives 10mL x 2

For non-invasive fetal aneuploidy screening (NIPS) tests using maternal blood and circulating tumour DNA testing in oncology. Avoid possible backflow from the tube. Tube contents should not touch stopper or the end of the needle during the collection procedure.

Order of Draw: Perform Cell-Free DNA BCT tubes first. After the tube is filled completely, gently invert 8 to 10 times.

PLAIN STERILE UNIVERSAL BOTTLE For Amniotic Fluid Karyotyping or QF-PCR
SODIUM OR LITHIUM HEPARIN BLOOD TUBE For Karyotyping and FISH
EDTA BLOOD TUBE For majority of Molecular Genetics tests
Miscellaneous Supplies
TYPE OF SUPPLIES/DESCRIPTIONCOMMENTS
CAPILLARY TUBE (Heparinised)

Self sealing

For neonatal serum bilirubin test. Collect 2 or 3 capillary tubes (heparinised) of blood.

SYRINGES and NEEDLES

Disposable syringes & needles

For collecting blood into non-vacutainer tubes, i.e. conventional screw-top containers.

Sizes available for syringes: 20mL, 10mL, 5mL & 2.5mL

Sizes available for needles: 23G 1”, 22G 1½”, 21G

1½"

VACUTAINER NEEDLE

Green 21G vacutainer needle, regular size

Disposable vacutainer needle to be used together with vacutainer needle holder for drawing blood specimen into vacutainer tubes.
DISPOSABLE VACUTAINER NEEDLE HOLDER

White hollow plastic tube

Used to hold vacutainer needle and the vacutainer tube while collecting blood specimen.
OTHER SUPPLIES, include non-vacutainer collection tubes, thin prep vial with cytobrush etc. Please call 6278 9188 to request for other supplies.

Blood Collection Containers – Common Tubes

Please refer to our Order of Draw posters provided.

Obtain a hard copy poster for your clinic by contacting your respective account managers or the Call Centre.

Order of Draw - Vacutainer Method Order of Draw - Wingset Method Order of Draw - Syringe Method

Note: Failure to fulfill the requirements stated may result in specimen rejection.

Other Specimen Collection Containers
TYPE OF CONTAINERS/DESCRIPTIONCOMMENTS
eSwab with liquid Amies medium (sterile applicator swab with flocked nylon fiber tip)

For bacteriological culture from all sites.

It contains a special liquid transport medium which will maintain micro-organism viability but does not promote growth of micro-organisms.

Pink cap eSwab is suitable for both aerobic & anaerobic organisms.

Double eSwab (flocked swabs with Amies medium)
  1. Pink eSwab - Sample axilla and groin, swirl in Amies media and discard swab
  2. White eSwab - Sample the anterior nares, break swab and place it in the tube of Amies media
For Methicillin-resistant Staphylococcus aureus (MRSA) PCR - XPERT test.
DRY SWAB without Transport Media

Sterile cotton swab attached to plastic applicator stick

For Chlamydia Trachomatis PCR and Neisseria Gonorrhoeae PCR test.
SINGLE eSWAB (flocked swabs with Liquid Amies medium) For Vancomycin-resistant enterococci (VRE) PCR - XPERT and Carbapenem-resistant Organisms (CRO) PCR - XPERT Tests.
STOOL CONTAINER

Screw cap (brown) plastic container with attached spatula

For collecting stool specimen for routine examination and culture.
URINE CONTAINER, Sterile (UNIVERSAL CONTAINER)

Sterile plastic container with screw cap

For routine urinalysis and urine culture.

Also suitable for collecting body fluids and sputum for routine examination or culture.

Cytology / Histology
TYPE OF CONTAINERS/DESCRIPTIONCOMMENTS
CONTAINER, with Formalin

Clean container (50mL) half-filled with formalin

Supplied for transporting small histopathology specimens.

GLASS SLIDE

Rectangular 3”x1” glass slide with frosted end

Supplied for collecting cytology smears only. Please write patient’s name on the frosted end of slide in pencil.
SLIDE HOLDER

Blue or white plastic holder for 2 slide wells.

Supplied with spray fixative

Supplied for holding cytology glass slides only.
For Genetics Department
TYPE OF SUPPLIESCOMMENTS
BOTTLE OF TRANSPORT MEDIUM

For QF-PCR and Karyotyping on Chorionic Villus or Products of Conception

BOTTLE OF TRANSPORT MEDIUM For Bone Marrow Karyotyping

MOTTLED BLACK/TAN

Cell-Free DNA

BCT has chemical additives 10mL x 2

For non-invasive fetal aneuploidy screening (NIPS) tests using maternal blood and circulating tumour DNA testing in oncology. Avoid possible backflow from the tube. Tube contents should not touch stopper or the end of the needle during the collection procedure.

Order of Draw: Perform Cell-Free DNA BCT tubes first. After the tube is filled completely, gently invert 8 to 10 times.

PLAIN STERILE UNIVERSAL BOTTLE For Amniotic Fluid Karyotyping or QF-PCR
SODIUM OR LITHIUM HEPARIN BLOOD TUBE For Karyotyping and FISH
EDTA BLOOD TUBE For majority of Molecular Genetics tests
Miscellaneous Supplies
TYPE OF SUPPLIES/DESCRIPTIONCOMMENTS
CAPILLARY TUBE (Heparinised)

Self sealing

For neonatal serum bilirubin test. Collect 2 or 3 capillary tubes (heparinised) of blood.

SYRINGES and NEEDLES

Disposable syringes & needles

For collecting blood into non-vacutainer tubes, i.e. conventional screw-top containers.

Sizes available for syringes: 20mL, 10mL, 5mL & 2.5mL

Sizes available for needles: 23G 1”, 22G 1½”, 21G

1½"

VACUTAINER NEEDLE

Green 21G vacutainer needle, regular size

Disposable vacutainer needle to be used together with vacutainer needle holder for drawing blood specimen into vacutainer tubes.
DISPOSABLE VACUTAINER NEEDLE HOLDER

White hollow plastic tube

Used to hold vacutainer needle and the vacutainer tube while collecting blood specimen.
OTHER SUPPLIES, include non-vacutainer collection tubes, thin prep vial with cytobrush etc. Please call 6278 9188 to request for other supplies.